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Effectiveness of clinical surface cleaning and disinfection: evaluation methods
Marcos Antonio Ferreira Júnior
Revista Brasileira de Enfermagem
Objective: To discuss the methods employed to evaluate the effectiveness of clinical surface cleaning and disinfection (C&D). Method: This is a theoretical reflection based on scientific studies and the experience of the authors. Knowledge and current gaps, the need for further studies, and practical application of the methods were approached. Results: There are four main methods used to evaluate the effectiveness of clinical surface C&D: visual inspection, fluorescent markers, microbiological cultures, and adenosine triphosphate (ATP) bioluminescence. The first two are used to evaluate the process and to predict adherence to protocols by the staff, and the last two are employed to evaluate the results, therefore being the most relevant to assess the risk of infection. Final considerations: The ideal method was not found, because all of them showed limitations. There is a need for strategies to optimize the precision of these methods.
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Efficiency of cleaning and disinfection of surfaces: correlation between assessment methods
Prof. Dr. Adriano Menis Ferreira
Revista brasileira de enfermagem
to assess the correlation among the ATP-bioluminescence assay, visual inspection and microbiological culture in monitoring the efficiency of cleaning and disinfection (C&D) of high-touch clinical surfaces (HTCS) in a walk-in emergency care unit. a prospective and comparative study was carried out from March to June 2015, in which five HTCS were sampled before and after C&D by means of the three methods. The HTCS were considered dirty when dust, waste, humidity and stains were detected in visual inspection; when ≥2.5 colony forming units per cm2 were found in culture; when ≥5 relative light units per cm2 were found at the ATP-bioluminescence assay. 720 analyses were performed, 240 per method. The overall rates of clean surfaces per visual inspection, culture and ATP-bioluminescence assay were 8.3%, 20.8% and 44.2% before C&D, and 92.5%, 50% and 84.2% after C&D, respectively (p<0.001). There were only occasional statistically significant relationships between methods. the methods d...
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The Hygienic Effectiveness of 2 Different Skin Cleansing Procedures
Anna-Clara Rönner
Journal of Wound, Ostomy & Continence Nursing, 2010
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Evaluation of the Cleaning Procedure Efficacy in Prevention of Nosocomial Infections in Healthcare Facilities Using Cultural Method Associated with High Sensitivity Luminometer for ATP Detection
Angelo Baggiani
Pathogens, 2018
In healthcare facilities, environmental surfaces may be a reservoir of infectious agents even though cleaning and disinfection practices play a role in the control of healthcare-associated infections. In this study, the effectiveness of cleaning/disinfection procedures has been evaluated in two hospital areas, which have different risk category classifications. According to the contract with the cleaning service, after the daily ambulatory activities, the housekeeping staff apply an alcohol-based detergent followed by a chlorine-based disinfectant (2% Antisapril, Angelini; 540 mg/L active chlorine), properly diluted and sprayed. The contract provides for the use of disposable microfiber wipes which must be replaced with new ones in each health out-patient department. Surface contamination was analyzed using cultural methods and ATP detection, performed with a high-sensitivity luminometer. The values 100 CFU/cm2 and 40 RLU/cm2 were considered as the threshold values for medium-risk c...
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Correlation Between Surface Cleaning and Disinfection Methods in an Emergency Room
Prof. Dr. Adriano Menis Ferreira
2021
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Correlation between cleaning/disinfection surface monitoring methods in primary health care
Denise de Andrade
2017
800x600 ABSTRACT Objective : to evaluate the correlation between the monitoring methods (visual inspection, adenosine triphosphate, and colony forming units) of the process of cleaning and disinfecting surfaces of a Family Health Strategy. Method : this is a prospective, analytical study with a quantitative approach. Data analysis was driven by Spearman correlation tests , Fisher exact test and receiver operating characteristic (ROC) curve. The choice of surfaces with a high frequency of contact with patients or professionals was chosen . Correlation evaluations considered the results of cleaning monitoring methods before and after performing cleaning/disinfection of five surfaces. Results : a significant correlation between ATP quantification and aerobic microbial counts was observed only for the patient´s bed surface (r=0.649, P=0.001). The accuracy of the visual inspection was low, so it would not be the ideal method to evaluate surface cleaning. Conclusion : there was a signific...
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Monitoring of surface cleaning and disinfection in a Brazilian pediatric unit
Odinéa Batista
Therapeutic advances in infectious disease, 2023
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Limitations of the Efficacy of Surface Disinfection in the Healthcare Setting
Ian Hosein
Infection Control and Hospital Epidemiology, 2009
We examined the efficacy of 2 commercially available wipes to effectively remove, kill, and prevent the transfer of both methicillin-resistant and methicillin-susceptible Staphylococcus aureus from contaminated surfaces. Although wipes play a role in decreasing the number of pathogenic bacteria from contaminated surfaces, they can potentially transfer bacteria to other surfaces if they are reused.
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Monitoring the effectiveness of surgical handwashing in healthcare workers of a fourth level operating room using bioluminescence assays
Ingrid Carolina Guayan Ardila
Ingeniería Solidaria
Aims: To assesses the effectiveness of surgical handwashing (S-HW) in healthcare workers (HCWs) in terms of organic material and bacteria elimination by means of bioluminescence assays. Methods: 62-HCWs were subjected to bioluminescence analyses to determine adenosine triphosphate (ATP) and colony forming units (CFU) of Enterobacteriaceae (EB) and E. coli before and after S-HW. A checklist to evaluate adherence to hand hygiene (HH) protocol was implemented. Results: ATP before HH (510.9/RLU) was reduced after S-HW (54.6/RLU), evidencing a significant decrease (p=0.001). Regarding the bacteria, 26.6/CFU and 4/CFU for EB and E. coli were reported before S-HW, respectively. After S-HW, CFU decreased for EB (21.8/CFU) and E. coli (3.5/CFU) without showing a significant reduction (p=0.679). Low ATP did not evidence a direct relation with EB (p=0.082) and E. coli (p=0.680) reduction. Surgical instrument professionals (p=0.001), general surgeons (p=0.001), residents (p=0.017), orthopedists...
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Can adenosine triphosphate be a proxy measure in evaluation of hand disinfection effect?
Ole Kleiven
Journal of Hospital Infection, 2020
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